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Demonstration of Bacillus cereus in Orthopaedic-Implant-Related Infection with Use of a Multi-Primer Polymerase Chain Reaction-Mass Spectrometric AssayReport of Two Cases
Phillip H. Gallo, PhD1; Rachael Melton-Kreft, BS, RN1; Laura Nistico, PhD1; Nicholas G. Sotereanos, MD1; Jeffrey J. Sewecke, DO1; Paul Stoodley, PhD2; Garth D. Ehrlich, PhD3; J. William Costerton, PhD1; Sandeep Kathju, MD, PhD1
1 Center for Genomic Sciences, Allegheny-Singer Research Institute (P.H.G., R.M.-K., L.N., J.W.C., and S.K.) and Department of Orthopaedic Surgery (N.G.S. and J.J.S.), Allegheny General Hospital, 320 East North Avenue, Pittsburgh, PA 15212. E-mail address for S. Kathju: skathju@wpahs.org
2 National Centre for Advanced Tribology at Southampton (nCATS), School of Engineering Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom
3 Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, 320 East North Avenue, Pittsburgh, PA 15212
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Disclosure: One or more of the authors received payments or services, either directly or indirectly (i.e., via his or her institution), from a third party in support of an aspect of this work. In addition, one or more of the authors, or his or her institution, has had a financial relationship, in the thirty-six months prior to submission of this work, with an entity in the biomedical arena that could be perceived to influence or have the potential to influence what is written in this work. No author has had any other relationships, or has engaged in any other activities, that could be perceived to influence or have the potential to influence what is written in this work. The complete Disclosures of Potential Conflicts of Interest submitted by authors are always provided with the online version of the article.

Investigation performed at Allegheny General Hospital, Pittsburgh, Pennsylvania

Copyright © 2011 by The Journal of Bone and Joint Surgery, Inc.
J Bone Joint Surg Am, 2011 Aug 03;93(15):e85 1-6. doi: 10.2106/JBJS.J.01181
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Extract

Infection following primary total joint replacement occurs with a prevalence of approximately 1% to 2%, but it can be a devastating complication1. Rates of infection following revision arthroplasty are higher, approaching 10%2,3. The medical and economic costs of periprosthetic joint infection are expected to become greater in the future, with the number of primary total hip arthroplasties expected to grow by 174%, to 572,000 annually in two decades4.
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    These activities have been planned and implemented in accordance with the Essential Areas and policies of the Accreditation Council for Continuing Medical Education (ACCME) through the joint sponsorship of the American Academy of Orthopaedic Surgeons and The Journal of Bone and Joint Surgery, Inc. The American Academy of Orthopaedic Surgeons is accredited by the ACCME to provide continuing medical education for physicians.
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